2.5. Acclimatization of plantlets
Regenerated plantlets were directly planted in pots (7 cm diameter
perforated at the bottom) containing unsterilized sand, soil and vermiculite
in the ratio 1:1:1 (v:v:v). Three batches were made and subjected
to different conditions. The first batch was left in the mist house for 24 h
before transferring to the greenhouse. The second batch was left in the
mist house for 8 h and subsequently transferred to the greenhouse. The
temperature of the greenhouse was regulated by a thermostat set at 15
°C minimum and 25 °Cmaximum; humidity was between 55 to 60% and
the potted plants were watered once in two days. However, despite the
fact that the temperature was set, the greenhouse overheated and temperature
rose above 35 °C. The third batch was transferred to a shade
house (with 70% shade, maximum temperature of 25 ± 2 °C and minimum
temperature of 15 ± 2 °C) under natural light conditions (during
the evening time) immediately after potting and spraying with water
until the soil mixture was damp. The plants were watered once in two
days. In each case, the frequency of survival was recorded after 4 and
8 weeks.