Determination of Fatmax and MFO
Fatmax is defined as the exercise intensity with the MFO. MFO is defined as the maximum amount of lipid oxida- tion observed during the incremental exercise test. Lipid oxidation rates were calculated through indirect calorime- try from gas exchange measurements by using Frayn’s equations. For each participant, the rates were calculated after smoothing the curve plotting lipid oxidation as a function of exercise intensity. A digital filter (40 s) was used to smooth the breath-by-breath fluctuations with a width that depended on the amount of noise in the data (Takagi et al., 2014). MFO and Fatmax were determined for each participant in the morning and evening.