Nitzschia sp. (Bacillariophyceae) was grown under temperature and photoperiods mimicking those,
typical during summer, spring/fall and winter conditions in the southern United States, and using five silicate
(Si) concentrations. In general, higher Si concentrations resulted in higher growth rates in summer
and spring/fall conditions and lower organic content. Si-deficient Nitzschia sp. had higher levels of neutral
lipid compared to those growing in Si replete media. Under summer conditions, the proportion of saturated
fatty acids (SFA) and monounsaturated fatty acids (MUFA) was relatively stable compared with
spring/fall and winter conditions, and the proportion of polyunsaturated fatty acids (PUFA) was low. In
the winter condition, SFA and MUFA showed a gradient of decreasing abundance while PUFA gradients
increased with increasing Si concentrations in the medium. Cumulative productivity (optimization of
growth and lipid content) would be best in the spring/fall but less so in the other conditions for this strain
of Nitzschia sp.