15 Using sterilized forceps, transfer shoots into a sterile container and rinse them 3 times with sterile distilled water.
16 Place one shoot under stereomicroscope and locate meristem. Cut each leaf primordia one after another till the meristematic dome becomes visible (Fig. 12, step 1,2, and 3). To do so, either use sterile scalpel(blade number 11) or needle.
17 Cut the base of the dome and transfer to meristem media (Fig. 12, Step 4).
18 Cover each tube with a plastic cap and seal with parafilm.
19 Label each tube with an Accession number, Date of introduction and Line number (record by writing on parafilm using long lasting marker).