Visualization of enhanced green fluorescent protein
(eGFP)
Transconjugants harbouring pIJ8655 were examined for
eGFP expression by growing in TSB supplemented with
thiostrepton (25 lg ml)1
) at 28C, 250 rev min)1 for
5 days. The culture was examined under a fluorescence
microscope (Olympus BX-51) using a mercury vapour
lamp with blue excitation (BP450–480 nm, DM500 and
barrier filter BA515).