The PCR amplification was carried
out in the Mastercycler (Eppendorf) in 20l reactions mixtures
containing: 2 U of Taq Polymerase (Fermentas), 2l of 10× PCR
buffer with (NH4)2SO4 (Fermentas), 1.5M of each forward and
reverse primer, 1.5mM of MgCl2, 0.2mM of each dNTP, 1.6l of
Bovine Serum Albumin (Fermentas), 10.1l of H2O and 1l of
diluted DNA.