After 45
days of culture, the biomass were collected and concentrated by filtration from
A. ovalisporum culture and by continuous centrifugation from M. aeruginosa
culture with a Sorvall SS-34/KSB rotor system from ThermoScientific (Waltham,
Massachusetts, USA). Finally, the concentrates were frozen at –80 1C and lyophilized
(Telstar cryodos 80 model).