The liquid phase of the
fermented broth was filtered and placed in vials for analysis of
ethanol concentration. A GC (Agilent 6890) fitted with split inlet
and flame ionization detector (FID) and an HP-FAAP polyethylene
glycol TPA column was used for quantitative analysis of the products.
The oven temperature varied from 70 to 200 C at different
ramps and hold-up times and the carrier gas was helium.
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