Materials and methods
Microalgae
Microalgae B. braunii race A was supplied by Antofagasta University
(Chile), where it was grown in pilot scale batch reactors
(Bazaes et al., 2012). Microalgae was harvested by centrifugation,
frozen and storaged until its utilization. Lipid-spent B. braunii
(LSBB) was produced using a Soxhlet extraction unit for 16 h, with
petroleum ether as solvent, at a solvent-sample ratio of 10:1.
Proximate analysis of LSBB was done according to Babayan et al.
(1978).
Co-substrates: WAS and glycerol
The WAS co-substrate was obtained from San Isidro sewage
treatment plant (Labranza, Chile). The Glycerol co-substrate was
obtained from a pilot scale plant for biodiesel production from
rapeseed oil (Gorbea, Chile).