The agar disk diffusion method was conducted for the determination
of antimicrobial activities of BCSO against L. monocytogenes,
S. enteritidis, S. aureus and E. coli. Briefly, 0.1 mL from
8 log CFU/mL bacterial suspension was spread onto Mueller
Hinton Agar (MHA) plates. Filter paper disks (6 mm in diameter)
impregnated with 10 lL of the undiluted oil were placed
on the inoculated plates. The plates, after remaining at room
temperature in laminar flow for 2 h, were incubated at 37 C
for 24 h. The diameters of the inhibition zones were measured
in millimeters. All tests were performed in triplicate.