It has been developed the production of asparaginase based on cloning of Aspergillus oryzae. The A. oryzae
asparaginase has been cloned and expressed in commercial relevant yields in A. oryzae. The A. oryzae is well known
for production of food enzymes. The A. oryzae asparaginase has pH optimum at pH 6–7 with good activity between pH 5 and 8, which may be the pH range for production of potato products like French fries and crisps (tested by Novozymes A/S). The optimum temperature of the asparaginase activity measured at pH 7 is 60 C (Novozymes A/S). However at temperatures above 60 C the enzyme activity decreases rapidly (Novozymes A/S) wherefore a temperature range between 40 and 60 C may be preferable (a linear increase in activity from 60% to 100% is seen within the temperature range 40–60 C) (Food Standards, 2007). In general, the degradation of the asparaginase enzyme increase with increasing temperature.