Amplification and sequencing of the gene for 16S rRNA
The gene for 16S rRNAwas partially amplified using primers 27f and
1522rN as described by Johnson (1994). Amplification and sequencing
of both strands of the amplified 16S rDNA fragment were performed
as described previously (Rau et al., 2009).
2.6. Amplification of the genes for cytotoxin K-1 and cytotoxin K-2
Amplification of cytK-1 and cytK-2 was performed using primers
CK1F, CK1R, CK2F and CK2R according to Guinebretiére et al. (2006