Treatments with water and 10 mmol/L lactic acid followed
by incubation for 48 h at 100 and 60 C, respectively,
gave the highest RS III yields and were used to study the
effect of autoclaving time on RS III formation. When water
was used as a solvent, it was possible to decrease the autoclaving
time to 15 min without any detrimental effect on
the formation of RS III (Fig. 1). However, with 10 mmol/L
lactic acid, the amount of RS III formed increased with
increasing autoclaving time, attaining a maximum value at
45 min autoclaving time (Fig. 1). The RS III yields declined
when the autoclaving time exceeded 45 min indicating the
effects of continuing acid and thermal hydrolysis of cassava
starch. These shorter autoclaving times, while giving highRS
III yields