Under the optimal conditions described above for multiplex
PCR, the amplification products of E. coli, Y. enterocolitica
and A. hydrophila were analysed using
template DNA of each organism respectively (Figure 5,
lane 1). Three DNA fragments amplified with sizes of
152 bp (the PCR product E. coli), 330 bp (the PCR product
Y. enterocolitica) and 685 bp (the PCR product
A. hydrophila) were well separated.