The
parasites after their recovery from the hosts were
washed in normal saline to free them from mucus.
Then they were fixed in hot 70% alcohol after
fixation the nematode parasites were preserved in
glycerin alcohol (glycerin: 70% alcohol, 1:3) and
were mounted in glycerin and glycerin jelly. Faecal
samples were collected in collection tubes
containing 10% formalin and were examined by
direct smear, flotation and sedimentation techniques
for the presence of eggs (Urquhart et al., 1996).