Microalgae are potential valuable materials for biofuels. Microalgae
with sequenced genomes and transgenic capabilities
are suitable for studying cellular processes to provide information
on the basic cellular processes and regulation involved
in the synthesis of the biofuel precursors, which will be useful
for the cultivation. Moreover, microalgae that can grow well in
the laboratory may be suitable for large scale cultivation under
different environments. However, this is not true of all microalgae.
Therefore, there is uncertainty about whether the information
obtained from the laboratory model species can be applied
to mass cultivation outdoors. Numerous factors influence
the cultivation of microalgae, including light, nutrient supply,
CO2, pH, temperature and O2 [28–31]. It is important to apply
the factors that influence the cultivation of microalgae in the
laboratory to mass cultivation outdoors. The growth rate and
lipid content of microalgae have a close relationship with light,
nitrogen, phosphorus, and temperature [15,32,33]. The conditions
for growth and lipid accumulation are different with different
species. Therefore, the optimum mass cultivation conditions
to be used for microalgae should be those that increase
growth rate and lipid content, which are the main factors affecting
the biofuel process