To determine the protein and energy requirements in white grouper, the factorial approach is applied here. Quantification of energy and protein requirement in the growing fish is the sum of the needs for maintenance and growth. The energy
requirement for maintenance is mainly dependent on body size and temperature and therefore is proportional to the metabolic body weight. The requirement for growth is dependent on the amount and composition of the added gain. Thus, dietary intake can be calculated by including the utilization efficiencies for deposition of new growth.