A smear of the selected strain (A-4) was
prepared on a clean glass slide and the
smear was allowed to air-dry and then heatfixed.
The heat-fixed smear was flooded with
crystal violet and after one minute, it was
washed with water and flooded with mordant
Gram’s iodine. The smear was decolorized
with 95 % ethyl alcohol, washed with water
and then counter-stained with safranin for 45
s. After washing with water, the smear was
dried with tissue paper and examined under
oil immersion (100 x) [10]