Field experiment were conducted on Vitis vinifera L,at the vineyard in Bhandgaon village Pine, located on the western peninsula of India in the state of Maharashtra. Ametoctradin 27% SC and Dimethomorph 20.3% SC was applied at recommended dose. The fungicide were sprayed with 1000/ha^-1 water during March 2005. The treatment, including the untreated control were replicated 3 time. After 2 h of spaying, the grapes samples were collected for analysis by image acquisition and processing. Image of grapes bunches were taken with a digital camera FOR SIX for consecutive days. At the end of the experiment, grape samples were subjected to pesticide residue analysis using LC-MS/MS.