Waste bones were collected from local slaughter houses, butchers,
and restaurants. The bones were manually cleaned from meat
pieces, repeatedly washed, and subsequently boiled in distilled
water for 4 h in order to remove fats. The cleaning process was
repeated 4 times. Thereafter, the clean bones were dried at
100 C for 8 h. The dried bones were crushed, milled, and sieved
into 4 different particle size ranges which were (>0.075), (0.075–
0.30), (0.3–1.18) and (1.18–2.34) mm for the powder, small, medium,
and large size-particles, respectively.