During the Double-Ovsynch treatment, the preOvsynch was done as follows: 100 μg of GnRH(d 58 ± 3; 2 mL of Cystorelin , Merial Ltd., Duluth, GA), followed by an injection of PGF2α (5 mL of Lutalyse; Pfizer Animal Health, New York, NY) 7 d later and GnRH 72 h after PGF2α.