Bands resulted from this method have such sizes that remove
the need for the use of polyacrylamide for electrophoresis.
Therefore, with the increase in the voltage during the use of
agarose gel, electrophoresis time is also decreased. When using in vitro cultivation routine methods, it is probable that in the
most cases the PCR test shows false negative results due to the
low number of pathogenic bacteria in water (less than detectable
by culture) or the loss of chromosomes of bacteria at different
stages of purification, in addition to being energy consuming of
routine methods of in vitro cultivation[16]. In molecular methods,
detection scope is much less than medium and bacteria are
detected even in small number. On the other hand, the only
existing way to detect VBNC is molecular methods. In this study,
with the aim of reducing the false negative responses, samples
filtration was used based on the method proposed by WHO.