2. Materials and methods
2.1. Experimental animals
Mature female freshwater prawns, M. rosenbergii (42.71±5.82 g
body weight) were obtained from commercial farms in Chonburi
Province, Thailand. The animals were kept in a 1400 L circular
concrete tank (80cm depth, 150 cm diameter). About 30% of the
water was changed every 2 days. Prawns were fed commercial
food pellets twice per day. Aeration was given all day. Thirty small
plastic cages were added in the tank for molting animals to hide