The wilt development on each tomato plant was rated as described (Bora et al., 2004): 0 = no symptoms; 1 = <25% of leaves with symptoms; 2–26–50% of leaves with symptoms; 3 = 51–75% of leaves with symptoms; 4 = 76–100% of leaves with symptoms. The per cent disease index was calculated as follows: Disease index = (∑(rating × number of plants rated)/Total number of plants × highest rating) × 100.bMean of three replications.cOne unit of the enzyme was defined as the amount of enzyme that will release 1.0 μmol of p-nitrophenol from the appropriate substrate per min at pH 4.8 at 37 °C.
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