The titers of IgG subtypes IgG1 and IgG2c were also analyzed.
The adjuvant and saline control mice did not have any detectable
levels of either IgG1 or IgG2c specific for Sm-Cathepsin B. In the
mice immunized with the recombinant protein and the adjuvant,antigen specific IgG1 titers started to rise at week 3 and the mean
endpoint titers achieved before cercarial challenge at week 9 were
9840 (Fig. 3b). Antibody levels of Sm-Cathepsin B specific IgG2c
were also detected in the mice immunized with the protein in the
presence of adjuvant. Titers for this IgG subtype were more robust
than those observed for IgG1. IgG2c titers began to rise at week 3
and reached the highest level at week 9 before the cercarial challenge
(Fig. 3c). The mean IgG2c endpoint titers at week 9 were
61,440