tDiabetes mellitus is a worldwide health problem affecting 1–2% of the population of world with noticeablemorbidity and mortality. Vascular events such as hypertension, nephropathy, neuropathy and retinopa-thy are happened in diabetic patients. Decline in tissue blood circulation may causes hypoxia and finallymay leads to slow wound healing and amputation. PVA/Chitosan Nano fiber wound dressings have highmoisture vapor transmission rate and good antimicrobial activity1PCNWD substrate does not haveany recognized cytotoxicity effects and has excellent odor absorbing capability. In the present study,Streptozotocin (STZ) is used to induce diabetes in rats, Skin ulcers are produced experimentally inthe experimentally induced diabetic and non-diabetic rats. Then PCNWD used as wound dressing for2 weeks period to evaluate its macroscopic and microscopic effects on wound healing in comparisonwith untreated diabetic and non-diabetic rats experimental ulcers. The findings of current study indicatesignificant acceleration in diabetes wound healing on the rats treated by PVA/Chitosan Nano fiber.