This study involved 40 Holstein-Friesian dairy cows [12 primiparous and 28 multiparous (mean parity, 2.4 ± 1.23 SD)], with cows having a mean PTA for milk yield and fat + protein yield of 238 (±136.6 SD) kg and 24.6 (±8.90 SD) kg, respectively, and a mean Profitable Lifetime Index (PLI) of £91 (±46.6 SD).