Fractionation was carried out by successive extraction with liquid
CO2 of pressure 62±1 bar. The lower part of the apparatus was kept
in the water bath at 35 ◦C to evaporate CO2. Cold water (5 ◦C) from a
thermostatic bath was circulated through the cooling finger of the
apparatus. Liquid CO2 placed in the lower compartment evaporates
at the bottom of the apparatus due to the heat supplied from the
warm water of the bath, gets condensed at the cold finger and the
liquid CO2 gets collected in the upper compartment of the Soxhlet
apparatus over the material to be extracted. When liquid CO2
gets filled up to the siphon level, the liquidCO2 soluble floral extract
siphons out to the lower receptacle kept in the lower compartment.