IVF took place in droplets (90L) containing tyrode albumin lactate pyruvate (TALP) supplemented with 6mg/mL fatty acid free BSA (Sigma–Aldrich), 100mMsodium pyruvate (Sigma–Aldrich), 75g/mL gentamicin (Sigma–Aldrich), 11g/mL heparin and 44g/mL PHE (2mM penicillamine, 1mM hypotaurine, 250mM epinephrine; Sigma–Aldrich)