Immunoassay has been widely used; however, a serious drawback
in the immunoassay of insulin is its nonspecific binding with
the coexistent biomolecules that cause imprecise detection of it [6].
In addition, high-performance liquid chromatography with ultravioletevisible
detection [4] and fluorescence detection [7] has been
used in this region, but it is time-consuming and an expensive
instrument. Hence, the development of a simple, rapid, sensitive,
and inexpensive method for insulin detection is still highly desirable.