Determination of Lactic Acid
The production of lactic acid was determined by titrating 10 ml of the homogenized sample against 0.25 mol/l NaOH using 1 ml of phenolphthalein indicator (0.5% in 50% alcohol). The titratable acidity was calculated as percentage lactic acid (v/v). Each millilitre of 1 N NaOH is equivalent to 9.008 mg of lactic acid (AOAC 1990)..