The optimum magnesium sulphate concentration was found to be 0.25%. [14] observed that the optimal medium for pullulan production by Rhodotorula bacarum was 8.0% (w/v) glucose, 2.0% (w/v) soybean cake hydrolysate, 0.5% (w/v) MgSO4.7H2O, and 0.06% (NH4)2SO4, pH 7.0. Calcium chloride can control pH of the fermentation medium and has its’ own effect on growth. In the present investigation, presence of 0.15% calcium chloride in the medium was most favourable for growth.