Study of beef cattle supply chain project in Chumphon and Prachuap Khiri Khan province has the objective to study the status of production and marketing of beef and beef cattle. It also has the aim to study the composition of supply chain activities, managements, marketing channels and cost and value-added activities of elements in the supply chain of beef and beef cattle in Chumphon and Prachuap Khiri Khan province. Pattern of the beef cattle production in both provinces, the most of farmers have 1-10 heads which be 78.90 percentage of the all farmers. In Chumphon provice we find the farmers have the most of other agricultural carrier along with beef cattle as palm oil planting which be 53.58 percentage and rubber planting as 18.54 percentage. The farmers in Prachuap Khiri Khan have the other carrier as coconut, pineapple and alovela planting. The most of beef cattle are hybrid cattle which come from internal breeding on farm or trading between local farmers by using native breed and Brahman. They are fed by grazing only natural grass as 45.01 percentages and roughage as corn, hay or grass. Feeding in the barn by the grass cutting is 15.11 and 14.70 percentages for the concentrate feeding. They feed by without the concentrate 80.75 percentages of the farmers The average of income from beef cattle feeding from both provinces is 433.29 baht per month. The most expenses are medical and artificial insemination cost which higher than other costs. The most of live beef cattle selling is by the middleman which own the butcher coming to farm. The price will be base on weight and condition of cattle. In some area of Prachuap Khiri Khan province the middleman will accumulate the live culling cattle and sale to the beef ball factory in Pathum Thani, Ang Thong and Ratchaburi as 42 baht per kilogram which the fattening beef cattle price as 46 baht per kilogram. The most selling in Chumphon area is for the middleman and butcher shop owner which slaughtering and sale in the province.