In order to bring a sufficient amount of reducing sugar liberated before the fermentation, 72 h -
saccharification was carried out on jackfruit seed starch medium by mono-culture of Amylomyces
rouxii MNT 037 and co-culture of A. rouxii MNT 037 and R. oryzae MNT 006 as primary
inoculum. After saccharification periods, the fermentation process began after the addition of the
secondary inoculum of S. cerevisiae YRK 017 for 96 h. This study process was applied from the
procedure of traditional alcoholic beverage that made from Loog- pang. The results showed that
ethanol production by SHF process with mono-culture of A. rouxii MNT 037 (18.98 gL-1) was
higher than that of other treatments (Table 2).