From the results we can conclude that planting Lavendula multifida ‘Spanish Eyes’ at 15 °C is the most optimum for lavender production. It showed the highest of plant height and peduncle length. Temperature at 25 ºC is not suitable for lavender growing although it gave earlier flowering. Therefore we could suggest that Lavendula multifida ‘Spanish Eyes’ should be planting at the highlands of Northern Thailand which have an optimum temperature.