The best (p≤0.05) survival rate of live, intact spermatozoa was in the pellet method and slow programmable protocol with 10% EG with 28.6 and 23.5%, respectively. The efficiency of the fast programmable protocol was rather low in both cases (12 and 15.5%), slow protocol with 6% DMF (11.4%) whereas freezing in nitrogen vapor produced the lowest survival rate (7.9 and 8.6%), irrespective of the type of CPs (Fig. 2).