Size exclusion chromatography analysis was used to evaluate
the average molecular weight (Mw) and polydispersity (Mw/Mn) of
the obtained lignin samples. N,N Dimethylformamide (DMF) eluent
was used as GPC mobile phase, at a flow rate of 0.7 mL/min
and 35 ◦C, using a Jasco Inc., chromatograph provided with an LCNetII/
ACD interface, a column oven CO-2065 Plus and a RI-2031
Plus Intelligent Refractive Index Detector. A guard column and two
columns PolarGel-M (Varian Inc.) were employed. Calibration was
made using polystyrene standards provided by Fluka, ranging from
250 to 70,000.