Inbred BALB/c mice, 6–8-weeks-old, were immunized according to the method described by Anuracpreeda et al. (2006).
The hybridoma clones expressing MoAb against rFgCatB3 were produced by fusing the splenocytes of immunized mice with
non-secreting mouse myeloma cells (P3x63-Ag8.653) using polyethylene glycol (Sigma–Aldrich Inc., St. Louis, MO, USA).