The sucker explants were soaked in 100% Clorox and three drops of Tween-20 for 15 minutes under
laminar flow. The explants were rinsed with sterilized distilled water for three times, followed by soaking in
70% ethanol for 5 minutes. As the final step, the suckers were rinsed by sterilized distilled water for three
times, and were trimmed, cut and cultured in MS media [13].