The enriched cultures were diluted
and incubated in nutrient agar plates (30C); then they were streaked on to the
Rhodamine B-olive oil agar plate containing 28 g/l nutrient agar, 4 g/l sodium
chloride, 10 ml Rhodamine B solution (1 mg/ml) and 31.25 ml of olive oil, and
incubated at 30C for two days. While the plates were irradiated with UV light, the
presence of orange fluorescent halos was visualized around the colonies. The bacteria
showing large fluorescence halos were selected as potential lipase producers.
These organisms were isolated and sub-cultured in a nutrient broth containing olive
oil (200 mg/l). The strain which showed the maximum lipase activity was identified