Four actual urine samples taken from young persons (male and female) who were suspicious to consumption of amphetamines were also subjected to the proposed procedure of DLLME-SFO, and analyzed in triplicate. All of urine samples analyzed within 24 h of collection. AP and MA were detected in all of the actual urine samples in the range of 46.1–166.4 and 85.6–320.5 g l−1,respectively. The concentration of AP and MA in different actual urine samples is shown