Climate variability of the world have negative impact on physiological processes of many of the world's grains, especially soybeans. Thus, the purpose of the research was to study the effects of different levels of temperature on nitrogen accumulation in different tissues; roots, stems and leaves of two Thai soybean cultivars (glycine max (l.) Merrill). In a study conducted using area on Converted Agricultural Research at the Naresuan University, Phitsanulok. Soybean sample were exposed to 3 levels of air temperature; Lower air temperature (LT), Higher air temperature (HT) and Control air temperature (CT), Uses electronic controls system, from 8:00 to 17:00 pm. By controlling the time of occurrence of weather conditions that different from the abnormal state for about 70% of the planting season. From V3 stage up to harvest under open top chamber system. During the trial operation showed that the temperature inside the cabinet can control the temperature averaged 25.5 C, 36.9 C and 35.0 C in treatment LT, HT and CT, respectively. The study found that Soybean planting under conditions increased temperatures or lower affect the accumulation of nitrogen in the tissues; roots, stems and leaves of two Thai soybean varieties different on stages of development.