thawwg. Further, it does not require labor-intensive exercises, such as the linear precooling and rapid thawing (Abe
1977 Yokoyama 1977, 1992 Smith 1988, 1991; Ito 1991
Smith and Onions 1994 Ito and Nakagiri 1996 0hmasa et
al. 1992, 1996) that is indispensable for the usual storage by
deepfreezing for reliable preservation. Tbese results sug-
gest that the sawdust-freezing method with usage of a
cryoprotectant is one of the most suitable metbods com-
pared to other ffeezing methods using agar disks.