Investigating the action of urease is project explains the importance of the enzyme urease and presents a protocol to demonstrate urease activity in the classroom. Enzymes play an essential role in the metabolism of all organisms. They catalyse and control most biochemical reactions in our body – from the replication of genetic information (DNA polymerase) to digestion. Enzyme activity, however, is not always easy to visualise. This is a simple and cheap practical protocol to help teach the topic of enzyme activity in the classroom. All of the required materials are readily available and safe. The urea Was discovered Friedrich Wöhler. Which until the living organisms. subsequently, Urease from jack beans was the first enzyme ever purified and crystallized by James B. Sumner.The urease enzyme the hydrolysis of urea to carbon dioxide and ammonia.And can be found in seeds, micro-organisms, vertebrates, bacteria and plants. The testing pH of urease enzyme use citric acid, Sodium bicarbonate and red cabbage extract on the indicator .At pH 7, the solution is violet/blue. If the solution turns red, pH less than 7. When the pH rises above 7 and the solution is more alkaline, the extract turns green.