The SBT-E1 cells were cultured, total lipid was extracted and the
different classes of lipids were separated as described above. The separated
lipids were visualised by spraying the plates with fluorescein 5-
isothiocyanate in methanol and the areas of silica gel corresponding to
the different lipid classes were scraped from the plates into separate
vials. Fatty acid methyl esters (FAME) were produced from the different
lipid classes and analysed by gas chromatography as previously
described (Gregory et al., 2011).