storage, bacterial populations were near the limit of detection by plating
(10 CFU/nut) and average counts were estimated using a combination
of plate counts and enrichment data. A range of bacterial levels was observed
among samples within a single time point, sometimes resulting
in a standard deviation that was larger than the average counts, in
part, a product of the combination of enumerated and assigned values
for samples (Table 2). This is an inherent limitation of microbiological
data. Gathering statistically sound plate count data is only possible
when using higher inoculum concentrations, but such treatments are
less likely to mimic natural contamination scenarios. Lowering the
limit of detection for enumeration by filtration or including MPN
determinations would add time and cost to the analysis but should be
considered for future studies. Differences among samples in nut shell topography
and shell integrity (e.g., small cracks) may also have contributed
to this variation by infl
uencing our ability to remove inoculated
organisms with our sampling procedure.
storage, bacterial populations were near the limit of detection by plating
(10 CFU/nut) and average counts were estimated using a combination
of plate counts and enrichment data. A range of bacterial levels was observed
among samples within a single time point, sometimes resulting
in a standard deviation that was larger than the average counts, in
part, a product of the combination of enumerated and assigned values
for samples (Table 2). This is an inherent limitation of microbiological
data. Gathering statistically sound plate count data is only possible
when using higher inoculum concentrations, but such treatments are
less likely to mimic natural contamination scenarios. Lowering the
limit of detection for enumeration by filtration or including MPN
determinations would add time and cost to the analysis but should be
considered for future studies. Differences among samples in nut shell topography
and shell integrity (e.g., small cracks) may also have contributed
to this variation by infl
uencing our ability to remove inoculated
organisms with our sampling procedure.
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