TSCE-E: TSCP was stirred in 30 mL of 70%
ethanol using a magnetic stirrer for 4 h and
centrifuged at 1500 rpm for 10 min, the clear portion
was collected. The residue was re-extracted with
30 mL aliquot of 70% ethanol until colourless. The
pooled extracts were filtered and concentrated to half
of its original volume. The resulting aqueous ethanol
extract was partitioned with an equal volume of
chloroform to discard lipid, and the upper layer of
aqueous ethanol was collected and partitioned with
ethyl acetate. The ethyl acetate layer was collected,
concentrated, and dried to obtain the dried extract,
designated as TSCE-E.