The fermentation medium is the same as the previously used
medium (Omeliansky’s medium) during isolation with the only
difference of addition of different carbon sources or different
nitrogen sources and adjustment of pH for optimization media
in different experiments. Different fermentable sugars or different
nitrogen sources have been shown to either induce or inhibit
cellulase production depending on individual species.To decipher
the sugar effect or the effect of nitrogen sources on C1 strain
cellulase activities of supernatant samples attained from cultures
grown on either glucose, cellobiose, CMC, lactose, sucrose,
or cellulose etc. as carbon sources and KNO3, (NH4)2SO4,
NH4NO3, NH4Cl, peptone etc. in medium were compared. Similarly
different pH was adjusted during medium preparation to
determine the optimumpHof C1 strain for cellulase production.
All the ingredients were mixed proportionately except carbon
source and the pH was adjusted by 0.2 N NaOH/0.1 N HCl.
Then the medium was sterilized by autoclaving at 121 C for
15 min. The carbon source was sterilized separately and added
to the fermentation medium during inoculation. To check the
maximum production of cellulase by the C1 strain in culture,
the medium was enriched with the supplements step by step
which individually increase the cellulase production.