2.1.1. Primary screening
All PNSB (earlier isolated from water and sediments from rice
paddy fields in Phatthalung, Nakhon Sri-Thammarat, and Songkhla
Provinces of Thailand) were screened to obtain isolates that grew
well in the presence of 0.25% NaCl (OD660 > 1.0), under both
microaerobic-light and aerobic-dark conditions. The concentration
of 0.25% NaCl in GA broth is equivalent to 4 mS cm1 electrical
conductivity, which matches the average of the saline paddy fields
in southern Thailand (Nunkaew et al., 2012)