Replacing soybean meal with tofu waste in antibiotic producing medium
Tofu waste was replaced with soybean meal in the original
antibiotic producing medium (APM) at the same rate (1:1). VN10-A-
44 was cultured on MAPM broth for 7 days at 30°C with shaking for
150 cycles/minute. The cultured broth (100 μl per well) was placed
on the test medium seeded with R2 with the method mentioned
previously in the materials and methods. After incubation for 24 to
72 h, the results were quantified by the diameter of the inhibition zone (cm).